Cell cycle periods in batch culture

Range Table - link
Organism Bacteria Escherichia coli
Reference Michelsen O, Teixeira de Mattos MJ, Jensen PR, Hansen FG. Precise determinations of C and D periods by flow cytometry in Escherichia coli K-12 and B/r. Microbiology. 2003 Apr149(Pt 4):1001-10.PubMed ID12686642
Method Researchers used flow cytometry to determine the cell cycle periods C and D in steady-state batch cultures of seven strains of E. coli K-12 and three of E. coli B/r (Table link). The different generation times were obtained by using different carbon sources. Flow cytometry can obtain multiparametric measurements of thousands of microscopic particles such as cells or chromosomes suspended in a stream of fluid per second.
Comments DNA distributions obtained by flow cytometry were resolved with researchers' computer software into the cell cycle periods C, D and B. Generation time range 21min (LJ24)-450min(FH1228). C period 39min (RH147)-105min (MG1655). B period-time between division time and initiation of replication of chromosome. C period range - time to replicate chromosome. D period-time between termination of replication and division time.
Entered by Uri M
ID 105065