| Value |
100
mM
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Moncany ML, Kellenberger E. High magnesium content of Escherichia coli B. Experientia. 1981 37 (8):846-7. abstract & p.847 table at bottom of left columnPubMed ID7026272
|
| Method |
"During the deposition of ceils from
an exponentially growing culture (1 ml of a 2x10^8 bacteria/ml suspension) on a membrane filter (Millipore
GSWP, 0.2 µm) under suction, the cells were maintained in
the presence of O2 and small amounts of nutrients." "The washed deposit of
cells on the membrane filter was then extracted with 1 M
HNO3, and the solution was analyzed by atomic absorption
spectrophotometry." |
| Comments |
"Results of equilibrium dialysis suggest that nearly all of this Mg is bound, probably most of it to nucleic acids." Table gives range of 90 - 110mM: "Concentrations calculated with cellular volumes of 1.4×10^-12 and 1.6×10^-12 ml" |
| Entered by |
Uri M |
| ID |
104983 |