DNA synthesis rate in presence of adenine and uridine

Value 1466 bp/min
Organism Chinese hamster ovary (CHO)
Reference Anglana M, Apiou F, Bensimon A, Debatisse M. Dynamics of DNA replication in mammalian somatic cells: nucleotide pool modulates origin choice and interorigin spacing. Cell. 2003 Aug 8 114 (3): 385-94. p.389 right column top paragraphPubMed ID12914702
Method "[Researchers] have a longstanding interest in the study of a specific chromosome subregion, amplified in Chinese hamster cells selected for resistance to coformycin, an inhibitor of adenylate deaminase2 (AMPD2)." "DNA from asynchronous cells was sequentially labeled with IdU and CldU." In DNA (chromosome) combing the DNA is stretched so that hybridization with probes is possible. By periodically adding radioactive label of different colors origins of replication and fork replication rate can be determined.
Comments "Analysis of combed DNA revealed that the average fork speed increased markedly (mean rate: 1466 bp/min) in cells grown in the presence of adenine and uridine (AU) (Figure 4A), while the doubling time (not shown) and S phase duration (Figure 4B) remained unchanged."
Entered by Uri M
ID 104936