| Range |
622-1287 bp/min
|
| Organism |
Chinese hamster ovary (CHO) |
| Reference |
Anglana M, Apiou F, Bensimon A, Debatisse M. Dynamics of DNA replication in mammalian somatic cells: nucleotide pool modulates origin choice and interorigin spacing. Cell. 2003 Aug 8 114 (3): 385-94. doi:10.1016/S0092-8674(03)00569-5 p.388 right column bottom paragraphPubMed ID12914702
|
| Method |
"[Researchers] have a longstanding interest in the study of a specific chromosome subregion, amplified in Chinese hamster cells selected for resistance to coformycin, an inhibitor of adenylate deaminase2 (AMPD2)." "DNA from asynchronous cells was sequentially labeled with IdU
and CldU." In DNA (chromosome) combing the DNA is stretched so that hybridization with probes is possible. By periodically adding radioactive label of different colors origins of replication and fork replication rate can be determined. |
| Comments |
"[Researchers] observed that fork speed progression was variable in each line (Figure 4A), as previously shown by others Housman and Huberman 1975 and Collins 1978. Again, the mean rate of fork progression was different in cells 422 and 471 (1285 and 1287 bp/min, respectively) and in cells 474 (622 bp/min). However, all three cell lines had comparable doubling times (˜18 hr not shown) and, as revealed by FACS analysis of BrdU-labeled cells, also displayed similar lengths for S phase (Figure 4B and data not shown)." |
| Entered by |
Uri M |
| ID |
104935 |