| Range |
Table - link
|
| Organism |
Human Homo sapiens |
| Reference |
Schoeberl B, Eichler-Jonsson C, Gilles ED, Müller G. Computational modeling of the dynamics of the MAP kinase cascade activated by surface and internalized EGF receptors. Nat Biotechnol. 2002 Apr20(4):370-5PubMed ID11923843
|
| Primary Source |
1) Berkers, J.A. van Bergen en Henegouwen, P.M. & Boonstra J. Three classes of epidermal growth factor receptors on HeLa cells. J. Biol. Chem. 266, 922-927 (1991). (2) Kholodenko, B.N., Demin, O.V., Moehren, G. & Hoek, J.B. Quantification of short term signaling by the epidermal growth factor receptor. J. Biol. Chem. 274, 30169-30181 (1999). (3) Starbuck, C. & Lauffenburger, D.A. Mathematical model for the effects of epidermal growth factor receptor trafficking dynamics on fibroblast responses. Biotechn. Prog. 8,132-143 (1992).PubMed ID1985972, 10514507, 1368006
|
| Method |
For receptors, Grb2 and coated-pit proteins the methods in Primary sources (1,2,3 respectively). For other proteins either estimation or the following biochemical quantitation: To determine the concentrations of signaling proteins Ras, MEK, ERK, and phosphorylated ERK by western blotting, researchers used the purified GST proteins of MEK, ERK, and ERK-PP (Upstate Biotechnology, Lake Placid, NY) and the His-tagged protein of c-H-Ras for the calibration curve. Cellular lysates of HeLa cells (for ERK and ERK-PP after stimulation with 50 ng/ml EGF) were run on the same gel. After blotting and detection of bands by the specific antibodies, the concentration of cellular proteins was calculated by a standard curve after densitometric evaluation. |
| Entered by |
Uri M |
| ID |
104828 |