| Method |
For cell diameter and nucleus/cytoplasm ratio measurements, a Zeiss Axiovert 200 microscope (Carl Zeiss, Thornwood, NY) with a Zeiss 100× 1.3 numerical aperture (NA) oil immersion phase objective was used. Nuclei were fluorescently stained with Hoechst 33342 (Molecular Probes, Eugene, OR) for visualization, and cell morphology was imaged with phase microscopy. Metamorph software (Molecular Devices, Downingtown, PA) was used to quantify the cross-sectional areas of the nuclei and whole cells. |