| Range |
Table - link
|
| Organism |
Various |
| Reference |
García-Pérez AI, López-Beltrán EA, Klüner P, Luque J, Ballesteros P, Cerdán S. Molecular crowding and viscosity as determinants of translational diffusion of metabolites in subcellular organelles. Arch Biochem Biophys. 1999 Feb 15 362(2):329-38PubMed ID9989943
|
| Method |
The role of molecular crowding and viscosity on the apparent translational diffusion coefficient (ADC) of small metabolites was investigated in different subcellular organelles using the pulse-field gradient spin-echo 1H NMR technique. ADCs of metabolites with increasing radius of gyration (0.7 Å < RG < 4.5 Å) were measured in the cytoplasm of rat or chicken erythrocytes, in the nucleus of chicken erythrocytes, and in isolated rat liver mitochondria. Metabolite ADCs in these systems were compared with the corresponding ADCs determined in model solutions of increasing bulk viscosity but different molecular crowding. |
| Comments |
Table also gives radius of gyration for the diffusing molecules |
| Entered by |
Uri M |
| ID |
104641 |