| Value |
3.61e+7
Copies/cell
|
| Organism |
Human Homo sapiens |
| Reference |
Thore A, Anséhn S, Lundin A, Bergman S. Detection of bacteriuria by luciferase assay of adenosine triphosphate. J Clin Microbiol. 1975 Jan1(1):1-8. Table - link PubMed ID1100645
|
| Method |
A selective method for distinguishing bacterial and nonbacterial adenosine triphosphate (ATP) in clinical bacteriological specimens was studied. The method involved incubation of samples with the detergent Triton X-100 and the ATP-hydrolyzing enzyme apyrase. The incubation selectively destroyed ATP in suspensions of various human cells while not affecting the ATP content in microbial cells. ATP remaining in the sample after incubation was extracted in boiling buffer and assayed by the firefly luciferase assay. |
| Comments |
Concentration of 6E-17 moles ATP/cell |
| Entered by |
Uri M |
| ID |
104453 |