Number of ATP molecules in Mononuclear white blood cells

Value 3.01e+7 Copies/cell
Organism Human Homo sapiens
Reference Thore A, Anséhn S, Lundin A, Bergman S. Detection of bacteriuria by luciferase assay of adenosine triphosphate. J Clin Microbiol. 1975 Jan1(1):1-8. Table - link PubMed ID1100645
Method A selective method for distinguishing bacterial and nonbacterial adenosine triphosphate (ATP) in clinical bacteriological specimens was studied. The method involved incubation of samples with the detergent Triton X-100 and the ATP-hydrolyzing enzyme apyrase. The incubation selectively destroyed ATP in suspensions of various human cells while not affecting the ATP content in microbial cells. ATP remaining in the sample after incubation was extracted in boiling buffer and assayed by the firefly luciferase assay.
Comments Equivalent to 5×10^-17 moles ATP/cell
Entered by Uri M
ID 104452