| Value |
87
min
Range: ±1 Table - link min
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Di Talia S, Skotheim JM, Bean JM, Siggia ED, Cross FR. The effects of molecular noise and size control on variability in the budding yeast cell cycle. Nature. 2007 Aug 23 448(7156):947-51 Supplementary online material p.18 table S7PubMed ID17713537
|
| Method |
P.947 left column bottom paragraph: "[Researchers] measured times from cytokinesis to budding (G1) and from budding to cytokinesis in haploids, diploids or tetraploids (mothers and daughters), using time-lapse fluorescence microscopy [a technique wherein a camera that periodically takes photographs is attached to a microscope, imaging cellular or intracellular activity] of strains expressing Myo1 tagged with green fluorescent protein (Myo1–GFP)." GFP (Green Fluorescent Protein), a fluorescing molecule, is often used as a label. In this case GFP was fused (through genetic engineering) to another protein, Myo1, which forms a ring at the daughter cell's bud neck. |
| Comments |
n=116. Although unspecified, the growth media appears to be glucose according to following sentence from p. 16 in supplementary information (bottom paragraph): "Glycerol/ethanol supports a much slower growth rate than glucose (170 min compared to 100 min doubling time)..." For doubling time of haploid cell from the same article see BNID 101310. See BNID 100270, 101747 |
| Entered by |
Uri M |
| ID |
104360 |