| Value |
600
bp
|
| Organism |
Human Homo sapiens |
| Reference |
Trinklein ND, Aldred SJ, Saldanha AJ, Myers RM. Identification and functional analysis of human transcriptional promoters. Genome Res. 2003 Feb13(2):308-12. p.309 left column 2nd paragraphPubMed ID12566409
|
| Method |
Researchers aligned full-length cDNA clones from the Mammalian Gene Collection to the human genome rough draft sequence to estimate the start sites of more than 10,000 human transcripts. Researchers selected genomic sequence just upstream from the 5' end of these cDNA sequences and designated these as putative promoters. Researchers assayed the functions of 152 of these DNA fragments, chosen at random from the entire set, in a luciferase-based transfection assay in four human cultured cell types. Ninety-one percent of these DNA fragments showed significant transcriptional activity in at least one of the cell lines, whereas 89% showed activity in at least two of the lines. |
| Comments |
To identify putative human transcriptional promoters, researchers aligned the sequences of the 10,276 full-length Mammalian Gene Collection (MGC) cDNA clones to the December, 2001 human draft sequence by using the BLAT algorithm (http://genome.ucsc.edu/cgi-bin/hgBlat?command=start). They then selected 600 bp of genomic sequence located -550 to +50 relative to the 5' end of each cDNA clone. If a cDNA is truly full length, they expected this sequence to include the basal promoter and nearby upstream regulatory elements. |
| Entered by |
Uri M |
| ID |
104335 |