| Value |
9e+6
M^-1×sec^-1
|
| Organism |
Generic |
| Reference |
Gorris HH, Walt DR. Mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies. J Am Chem Soc. 2009 May 6, 131(17):6277-82PubMed ID19338338
|
| Primary Source |
Chance B. The kinetics and stoichiometry of the transition from the primary to the secondary peroxidase peroxide complexes. Arch Biochem Biophys. 1952 Dec41(2):416-24.PubMed ID13008459
|
| Method |
Enclosing a population of hundreds of single enzyme molecules in a large array of femtoliter reaction chambers and observing the individual molecules by fluorescence microscopy |
| Comments |
Hydrogen peroxide (H2O2) initiates the peroxidase catalytic cycle (Figure 1) by a rapid (9 × 10^6 M^-1 s^-1) two-electron oxidation of the ferric ground-state HRP to Compound I which is a porphyrin-p-cation radical. |
| Entered by |
Uri M |
| ID |
104240 |