| Value |
710
sec^-1
|
| Organism |
Generic |
| Reference |
Gorris HH, Walt DR. Mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies. J Am Chem Soc. 2009 May 6131(17):6277-82PubMed ID19338338
|
| Method |
Enclosing a population of hundreds of single enzyme molecules in a large array of femtoliter reaction chambers and observing the individual molecules by fluorescence microscopy |
| Comments |
HRP first oxidizes fluorogenic substrate molecules like Amplex Red to radical intermediates. Two radical molecules subsequently undergo an enzyme-independent dismutation reaction |
| Entered by |
Uri M |
| ID |
104239 |