| Value |
540
proteins/mRNA
Range: 300 to 600 proteins/mRNA
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Lu P, Vogel C, Wang R, Yao X, Marcotte EM. Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation. Nat Biotechnol. 2007 Jan25(1):117-24. abstract, p.120 caption to figure 4b,e, p.121 right column 3rd paragraph and p.122 left column 4th paragraphPubMed ID17187058
|
| Method |
Abstract: "[Researchers] report a method for large-scale absolute protein expression measurements (APEX) and apply it to estimate the relative contributions of transcriptional- and translational-level gene regulation in the yeast and Escherichia coli proteomes. APEX relies upon correcting each protein's mass spectrometry sampling depth (observed peptide count) by learned probabilities for identifying the peptides." |
| Comments |
P.121 right column 3rd paragraph: "The E. coli proteome revealed a similar trend—APEX-based protein abundances correlated with average absolute mRNA abundances from at least two of three experiments [refs 30,31,32] (Rs = 0.69, R2 = 0.47, Fig. 4d). Again, the relationship is well described by a linear relationship [protein] = 540 × [mRNA], with ∼300–600 proteins/mRNA (Fig. 4e), depending upon details of normalization (Supplementary Notes and Supplementary Data 3). As with yeast, the logarithm of the ratio of proteins per mRNA is well fit (R2 = 0.94) by a normal distribution (Fig. 4f)." Note-Arkin et al 1998 give value of 10 proteins/mRNA, BNID 100196. |
| Entered by |
Uri M |
| ID |
104186 |