| Value |
5600
proteins/mRNA
Range: 4,000 to 7,000 proteins/mRNA
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Lu P, Vogel C, Wang R, Yao X, Marcotte EM. Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation. Nat Biotechnol. 2007 Jan25(1):117-24. abstract, p.120 caption of fig.4b,e and p.121 right column 2nd paragraphPubMed ID17187058
|
| Method |
Abstract: "[Researchers] report a method for large-scale absolute protein expression measurements (APEX) and apply it to estimate the relative contributions of transcriptional- and translational-level gene regulation in the yeast and Escherichia coli proteomes. APEX relies upon correcting each protein's mass spectrometry sampling depth (observed peptide count) by learned probabilities for identifying the peptides." |
| Comments |
P.121 right column 2nd paragraph: "Further, binning the measurements (Fig. 4b) indicates that the relationship between the numbers of protein and mRNA molecules has the form of a power law with an exponent close to one. Therefore, the distribution of individual proteins is also well fit by a linear relationship of [protein] = 5,600 × [mRNA], implying ∼5,600 proteins present per mRNA, which is somewhat higher than previous estimates [refs 20,35]. The logarithm of the ratio of proteins per mRNA is well modeled (R^2 = 0.98) by a normal distribution (Fig. 4c)." See BNID 106202 |
| Entered by |
Uri M |
| ID |
104185 |