| Value |
60
sec^-1
|
| Organism |
Human Homo sapiens |
| Reference |
Stein, Wilfred D., Transport and Diffusion across Cell Membranes, 1986 Academic press inc. Harcourt Brace Jovanovich, Publishers pp.557 Table - link |
| Primary Source |
Kaplan JH. Sodium pump-mediated ATP:ADP exchange. The sided effects of sodium and potassium ions. J Gen Physiol. 1982 Dec80(6):915-37.PubMed ID6294224
|
| Method |
Resealed human red cell ghosts containing caged ATP (Kaplan et al., 1978) and [3H]ADP were irradiated at 340 nm. The photochemical release of free ATP initiated a rapid transphosphorylation reaction (ATP:ADP exchange), a component of which is inhibited by ouabain. The reaction rate was measured by following the rate of appearance of [3H]ATP. Calculated assuming a stoichiometry of three sodium ions moved to two potassium ions moved to one ATP split per enzyme molecule in all modes. Data either appears directly in primary ref, or is calculation assuming 200 sodium pumps per cell |
| Comments |
At 37 degrees celsius |
| Entered by |
Uri M |
| ID |
104184 |