Sodium concentration in cytosol

Range ~5 mM
Organism Bacteria Escherichia coli
Reference Shabala L, Bowman J, Brown J, Ross T, McMeekin T, Shabala S. Ion transport and osmotic adjustment in Escherichia coli in response to ionic and non-ionic osmotica. Environ Microbiol. 2009 Jan11(1):137-48. doi: 10.1111/j.1462-2920.2008.01748.x. p.141 fig.3PubMed ID18793315
Method "non-invasive microelectrode ion flux (MIFE) measurement technique." "Escherichia coli Frag1 was maintained on Luria–Bertani agar. Culture was grown in side-arm flasks containing glucose minimal media (DM, Minimal Broth Davis, Difco) supplemented with 0.1% of glucose at 25°C with shaking (60 r.p.m.) until cells reached exponential growth phase (optical density OD540 = 0.3). The culture was used for the MIFE experiments, intracellular measurements and MP measurements." "Exponential phase cells were treated with different concentrations of NaCl (ranging between 1% and 10% with 1% increment 0.3–3.53 Os kg-1 range) and isotonic concentrations of sucrose for 1 h." Extracted visually from fig.3
Comments "Changes in internal Na+ content essentially mirrored those for K+ content, with a strong negative correlation (r=-0.94) between intracellular K+ and Na+ found in response to NaCl treatment (Fig. 3). The critical concentration appears to be 5% NaCl, after which the intracellular Na+ increased almost 10-fold, to the level of above 1 M (note the logarithmic scale in Fig. 3). Interestingly, at exactly that concentration (5%) the intracellular K+ level dropped below control values, as evident from Fig. 3. At the same time, no significant changes in internal Na+ content occurred in response to sucrose treatments for the entire range of concentrations tested." Under range of 0-3.5 Os/Kg external osmolality of sucrose in medium.
Entered by Uri M
ID 104050