||Bacteria Escherichia coli
||Shabala L, Bowman J, Brown J, Ross T, McMeekin T, Shabala S. Ion transport and osmotic adjustment in Escherichia coli in response to ionic and non-ionic osmotica. Environ Microbiol. 2009 Jan11(1):137-48. p.144 left column bottom paragraphPubMed ID18793315
||P.138 left column bottom paragraph: "....non-invasive microelectrode ion flux (MIFE) measurement technique..." P.145 left column 4th paragraph: "Escherichia coli Frag1 was maintained on Luria–Bertani agar. Culture was grown in side-arm flasks containing glucose minimal media (DM, Minimal Broth Davis, Difco) supplemented with 0.1% of glucose at 25°C with shaking (60 r.p.m.) until cells reached exponential growth phase (optical density OD540 = 0.3)."
||p.144 left column bottom paragraph: "Consistent with literature reports (Cayley et al., 1991. Asha and Gowrishankar, 1993. Jordan and Davies, 2001), [researchers] found intracellular K+ concentrations in
E. coli cells to range from 180 to 200 mM across the entire range of
sucrose concentrations employed (Fig. 3)." P.146 left column 2nd paragraph: "Exponential phase cells were treated with different concentrations of NaCl (ranging between 1% and 10% with 1% increment. 0.3–3.53 Os/kg range) and isotonic concentrations of sucrose for 1 h." See figure 3. K+ is the most abundant inorganic ion in the (non-halophilic) cell cytosol (a typical concentration of ~200 mM). See Kuo et al., 2005 PMID 16026885 p.962 right column 2nd paragraph:"K+ is the most abundant ion in cytoplasm. Escherichia coli, for example, has an internal K+ concentration of ∼200 mM, while the standard rich Luria–Bertani medium (LB) contains only ∼7 mM K+ (from the yeast extract added [ref 14])."