Potassium concentration in cytosol

Range 180-200 mM
Organism Bacteria Escherichia coli
Reference Shabala L, Bowman J, Brown J, Ross T, McMeekin T, Shabala S. Ion transport and osmotic adjustment in Escherichia coli in response to ionic and non-ionic osmotica. Environ Microbiol. 2009 Jan11(1):137-48. p.144 left column bottom paragraphPubMed ID18793315
Method P.138 left column bottom paragraph: "....non-invasive microelectrode ion flux (MIFE) measurement technique..." P.145 left column 4th paragraph: "Escherichia coli Frag1 was maintained on Luria–Bertani agar. Culture was grown in side-arm flasks containing glucose minimal media (DM, Minimal Broth Davis, Difco) supplemented with 0.1% of glucose at 25°C with shaking (60 r.p.m.) until cells reached exponential growth phase (optical density OD540 = 0.3)."
Comments p.144 left column bottom paragraph: "Consistent with literature reports (Cayley et al., 1991. Asha and Gowrishankar, 1993. Jordan and Davies, 2001), [researchers] found intracellular K+ concentrations in E. coli cells to range from 180 to 200 mM across the entire range of sucrose concentrations employed (Fig. 3)." P.146 left column 2nd paragraph: "Exponential phase cells were treated with different concentrations of NaCl (ranging between 1% and 10% with 1% increment. 0.3–3.53 Os/kg range) and isotonic concentrations of sucrose for 1 h." See figure 3. K+ is the most abundant inorganic ion in the (non-halophilic) cell cytosol (a typical concentration of ~200 mM). See Kuo et al., 2005 PMID 16026885 p.962 right column 2nd paragraph:"K+ is the most abundant ion in cytoplasm. Escherichia coli, for example, has an internal K+ concentration of ∼200 mM, while the standard rich Luria–Bertani medium (LB) contains only ∼7 mM K+ (from the yeast extract added [ref 14])."
Entered by Uri M
ID 104049