Effects of Adenylate Mixtures on NAD-Malic Enzyme activity
|Table - link
|Furbank RT, Agostino A, Hatch MD. Regulation of C4 photosynthesis: modulation of mitochondrial NAD-malic enzyme by adenylates. Arch Biochem Biophys. 1991 Sep289(2):376-81PubMed ID1898077
|NAD-malic enzyme was partially purified from bundle sheath cells of Atriplex spongiosa. Enzyme from Urochloa panicoides was purified according to Burnell (9) and appeared as a single band on SDS-polyacrylamide gels. NAD-malic enzyme activity was assayed spectrophotometrically as an absorbance increase at 340 nm in a 1-ml reaction as described in text. Enzyme was preincubated with the reaction mix for 1 to 2 minutes by malate addition prior to initiation of the assay by malate addition.