| Value |
-40.57
kJ/mole
Range: ±0.8 kJ/mole
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Masuda T, Sato K, Noda C, Ikeda KM, Matsunaga A, Ogura MN, Shimizu K, Nagasawa H, Matsuyama N, Izumi T. Protective effect of urinary trypsin inhibitor on myocardial mitochondria during hemorrhagic shock and reperfusion. Crit Care Med. 2003 Jul31(7):1987-92PubMed ID12847393
|
| Method |
The peak left ventricular systolic pressure was lowered to 50 mm Hg by bleeding, and this pressure was maintained for 60 mins by adjusting the volume of bleeding to induce hemorrhagic shock. Blood aspirated from the bleeding line was heparinized and stored at 4°C. After the hypovolemic period, the blood was warmed to 37°C and the rats were transfused using the whole volume of stored blood during a period of 10 mins. The animals were divided into two groups as follows: the UTI group was administered 50,000 units/kg/hr UTI by intravenous infusion over 10 mins before the start of bleeding and the control group was administered 2.0 mL/kg/hr physiologic saline solution instead. Gibbs free energy calculated according to Delta G(Phos) = Delta G° +RT×ln[ATP]/([ADP]×[Pi]) |
| Comments |
Value of 9.7±0.2 kcal/mol. Nucleotide and phosphate concentrations are [ATP]=3.1 µmol/g,[ADP]=0.12 µmol/g,[Pi]=0.017 µmol/g (extracted manually from figures 5 and 6 in ref). |
| Entered by |
Uri M |
| ID |
103965 |