Copy number of plasmids with ColE1 origin of replication
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Range | 50-70 copies/cell |
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Organism | Bacteria Escherichia coli |
Reference | Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15 25(6):1203-10 p.1205 table 1PubMed ID9092630 |
Method | The intracellular copy numbers were determined by comparing luciferase activity of cells harbouring the respective plasmids with the activity in cells containing only a single luciferase expression unit integrated in the chromosome (data not shown) |
Comments | Module II contains an origin of replication which is protected from outside transcriptional readthrough (ref 14 ) by two terminators. See figure 2 |
Entered by | Uri M |
ID | 103857 |