Kd for binding of Elongation Factor-G-GTP to vacant ribosome

Value 0.27 µM
Organism Bacteria Escherichia coli
Reference Yu H, Chan YL, Wool IG. The identification of the determinants of the cyclic, sequential binding of elongation factors tu and g to the ribosome. J Mol Biol. 2009 Feb 27 386(3):802-13.PubMed ID19154738
Method Preparation of ribosomes, [14C]Phe-tRNA, and N-Ac-[14C]Phe-tRNA, Formation of ribosome tRNA complexes, Chemical footprinting, Treatment of ribosome complexes with sarcin or PAP, Binding of EF-G to ribosome complexes
Entered by Uri M
ID 103826