| Value |
0.3
µm^2/sec
Range: ±0.1 µm^2/sec
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Gregor T, Wieschaus EF, McGregor AP, Bialek W, Tank DW. Stability and nuclear dynamics of the bicoid morphogen gradient. Cell. 2007 Jul 13 130(1):141-52 DOI: 10.1016/j.cell.2007.05.026 p.147 left column 2nd paragraphPubMed ID17632061
|
| Method |
P.147 left column 2nd paragraph: "To directly measure the diffusion constant of Bcd-GFP in the cortical cytoplasm, [investigators] measured the dynamics of recovery after pattern photobleaching. A rectangular volume 16 × 16 × 7 μm^3 in the anterior cytoplasm of Bcd-GFP-expressing embryos was photobleached during mitosis 13, when Bcd-GFP is uniformly distributed (locally) throughout the cortical cytoplasm and the cytoplasmic concentration is relatively high. A typical recovery curve is shown in Figure 5. Recovery curves were fit to solutions of the diffusion equation to estimate (see Experimental Procedures)." |
| Comments |
P.147 left column 2nd paragraph (continued from "Method" above): "From a total of 21 recovery curves in four embryos, [investigators'] analysis provided a cytoplasmic diffusion constant D = 0.30 ± 0.09 μm^2/s (mean ± SD) during mitosis 13, which is very close to [their] estimate from the transport model." |
| Entered by |
dandanbz |
| ID |
103811 |