| Value |
210
molecules/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Waygood EB, Steeves T., Can J Biochem. 1980 Jan58(1):40-8. Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties. Table 1PubMed ID6992959
|
| Method |
Preparation of Crude Extracts and Membranes, Assay of PTS Proteins and Enzymes, Sugar Phosphorylation Assay, Assays for HPr purification used methyl-alpha-[U-14C]-glucoside up to and including the Pellicon concentration, step. Polyacrylamide Gel Electrophoresis, Molecular Sieve Chromatography, Protein Determination, Sedimentation Velocity Studies, |
| Comments |
Equivalent to ~210 molecules/sec based on a MW of 135kD. Probably refers to the dimer state. Calculated from 0.000095 moles/min/mg (bion ). Multiply by 135000 Da and multiply by 1000mg/g, divide by 60 sec/min. 0.000095*135000*1000/60=213.75 molecules/sec. Calculated by Ron Milo |
| Entered by |
Uri M |
| ID |
103693 |