Time between decrease in NADPH and pollen tube growth
Range | 5-10 sec |
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Organism | Lily Lilium formosanum |
Reference | Cárdenas L, McKenna ST, Kunkel JG, Hepler PK. NAD(P)H oscillates in pollen tubes and is correlated with tip growth. Plant Physiol. 2006 Dec142(4):1460-8.PubMed ID17041030 |
Method | Pollen grains of lily (Lilium formosanum) were germinated and grown in lily growth medium (LGM), which is composed of 15 mM MES, 1.6 mM H3BO3, 1 mM KCl, 0.1 mM CaCl2, and 7% (0.2 M) Suc at pH 5.5. NAD(P)H is fluorescent and has been visualized by exciting at 340 to 365 nm and detecting emission at 400 to 500 nm |
Entered by | Uri M |
ID | 103671 |