| Value |
20
%
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Hani S. Zaher and Rachel Green, Fidelity at the Molecular Level: Lessons from Protein Synthesis. Cell Volume 136, Issue 4, 20 February 2009, Pages 746-762PubMed ID19239893
|
| Primary Source |
Blanchard SC, Gonzalez RL, Kim HD, Chu S, Puglisi JD. tRNA selection and kinetic proofreading in translation. Nat Struct Mol Biol. 2004 Oct11(10):1008-14.PubMed ID15448679
|
| Method |
Single-molecule fluorescence resonance energy transfer (smFRET). Intermediate states in the pathway of tRNA delivery were observed using antibiotics and nonhydrolyzable GTP analogs. Measured under relatively high magnesium, low fidelity conditions |
| Comments |
Initial selection is comprised of: Initial binding, coding recognition and GTPase activation |
| Entered by |
Uri M |
| ID |
103485 |