Metabolite sequestration by ribulose-1,5-bisphosphate carboxylase during steady-state photosynthesis under various llight regimes

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Organism Spinach Spinacia oleracea
Reference Karl-Josef Dietz and Ulrich Heber, Rate-limiting factors in leaf photosynthesis. I. Carbon fluxes in the Calvin cycle, Biochimica et Biophysica Acta, 767 (1984) 432-443 Table 3
Method Spinach was grown in a greenhouse with additional light in an 10 h light/14 h dark period. Leaves were harvested after more than 6 h illumination.Chlorophyll, the activity of pyruvate kinase and the distribution of ribulose 1,5-bisphosphate and uridine diphosphoglucose were taken to estimate the contamination of the chloroplast fraction by cytoplasm.
Entered by Uri M
ID 103461