Fraction of PolyP localized at the cell surface

Range 20-30 %
Organism Budding yeast Saccharomyces cerevisiae
Reference Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54.PubMed ID10739478
Primary Source Vagabov, V. M. (1988) Biosynthesis of Carbohydrate Components of Yeast Cell Wall [in Russian], NTsBI Akad. Nauk SSSR, Pushchino. AND Kulaev, I. S., Vagabov, V. M., and Shabalin, Yu. A. (1987) in Phosphate Metabolism and Cellular Regulation in Microorganisms (Torriani-Gorini, A., Rothman, F. G., Silver, S., Wright, A., and Yagil, E., eds.) American Society for Microbiology, Washington, pp. 233-238.
Method The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source. Then the cells are placed on the same medium except depleted of Pi under sterile conditions and the culture is grown during 7 h. Further, the cells are again placed on fresh complete medium, where the culture is growing for 2 and 4 h. The degree of polymerization (n) of polyP fractions was estimated from the areas of 31P-NMR-spectral peaks.
Comments The content of this polyP may comprise 20-30% of the total amount of cell polyP depending on the conditions of cultivation. See BNID 103450, 105839
Entered by Uri M
ID 103449