PolyP2 concentration in polyphosphates

Value 3800 µg P/g dry cell weight
Organism Budding yeast Saccharomyces cerevisiae
Reference Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54. p.351 Fig. 1(a)PubMed ID10739478
Method P.350 right column 4th paragraph: "Phosphate concentration was estimated according to Berenblum and Chain in the modification of Weil-Malherbe and Green (ref 16)." P.350 right column 5th paragraph: "To determine dry weight, aliquots of cell suspensions were applied on filters preliminary brought to a constant weight by drying under vacuum at 85°C and dried to a constant weight under the same conditions." P.350 right column 8th paragraph: "The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source (point A)." Extracted visually from Fig. 1. a).
Comments P.351 caption to Fig1.A: "PolyP [inorganic linear polyphosphate] content in the cells of S. cerevisiae under different conditions of their cultivation: PolyP content in the cells of S. cerevisiae under different conditions of their cultivation." P.351 right column bottom paragraph: "A comparison of polyP content in the yeast before phosphate starvation (point A) and after the transfer of the culture to the fresh complete medium (point C) indicates that super-accumulation is not characteristic for all polyP fractions. As seen from Fig. 1a, the maximal accumulation under the conditions in question is observed for the polyP3 (almost 5-fold) and polyP5 (more than 8-fold) fractions."
Entered by Uri M
ID 103433