Frequency of paternal chloroplast inheritance

Value 3E-06 Unitless
Organism Tobacco Nicotiana tabacum
Reference Ruf S, Karcher D, Bock R. Determining the transgene containment level provided by chloroplast transformation. Proc Natl Acad Sci U S A. 2007 Apr 24 104(17):6998-7002.PubMed ID17420459
Method To facilitate large-scale selection for occasional paternal transmission of transgenic chloroplasts, an experimental system consisting of (i) male sterile plants as maternal parents and (ii) plastid-transformed (transplastomic) lines as pollen donors that carry a plastid-localized antibiotic resistance gene and a reporter gene for visualization of paternally transmitted plastids was devised
Comments Plastids (chloroplasts) are maternally inherited in most crops. Maternal inheritance excludes plastid genes and transgenes from pollen transmission. The above value is the rate of contamination of genetically modified plants (contamination resulting in production of modified pollen due to paternal inheritance)
Entered by Uri M
ID 103098