Number of mitochondria in budding cell grown on glucose

Value 3.2 mitochondria/cell Range: Table - link mitochondria/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Visser W, van Spronsen EA, Nanninga N, Pronk JT, Gijs Kuenen J, van Dijken JP. Effects of growth conditions on mitochondrial morphology in Saccharomyces cerevisiae. Antonie Van Leeuwenhoek. 1995 67(3):243-53. p.247 table 1PubMed ID7778893
Method "[Researchers] used the vital stain for mitochondria methylpyridinium iodine (DASPMI), a fluorescent, non-toxic stain for mitochondria (Bereiter-Hahn 1976: Bereiter-Hahn et al. 1983), combined with confocal scanning laser microscopy (CSLM) (Brakenhoff et al. 1979, 1985, 1989). CSLM allows the generation of high resolution, three-dimensional images of living cells, without the disadvantage of conventional fluorescence microscopy: i.e., the strong out-of-focus fluorescence light which reduces the contrast of images made at high magnifications."
Comments "The most striking observation concerned the number of mitochondria, which was approximately ten-fold higher in ethanol-grown cells than in cells grown on glucose (Table 1). Although the number of mitochondria was different, the mitochondrial volume in ethanol-and glucose-grown cells was approximately the same (Table 1). This implies that mitochondria in ethanol-grown cultures have a much larger surface area than those in glucose-grown cells...In [researchers'] study, the different mitochondrial number and morphology observed in ethanol-and glucose-grown cells did not significantly affect the fraction of the cellular volume occupied by the mitochondria (6-7%, Table 1)." See notes above & beneath table. Note-Graziewicz MA et al., 2006 PMID 16464011 p.384 left column write that "Several hundred mitochondria can be present in individual cells."
Entered by Uri M
ID 103068