| Range |
10-100 unitless
|
| Organism |
Tobacco Nicotiana tabacum |
| Reference |
Svab Z, Maliga P. High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci U S A. 1993 Feb 190(3):913-7.PubMed ID8381537
|
| Method |
Tobacco suspension culture cells were bombarded with the recombinant DNA A constructs and selected for kanamycin resistance. |
| Comments |
plastid transformation in land plants is less frequent by 2-3 orders of magnitude than biolistic transformation of nuclear genes: one bombardment with DNA-coated microscopic tungsten particles yields 2-20 nuclear gene transformants (bion 102762), whereas one plastid transformant is recovered in about 100 bombarded samples |
| Entered by |
Uri M |
| ID |
102763 |