Carbonic Anhydrase III CO2 hydration turnover rate
| Value | <=8000 1/sec |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Lindskog S. Structure and mechanism of carbonic anhydrase. Pharmacol Ther. 1997 74(1):1-20.PubMed ID9336012 |
| Primary Source | Jewell DA, Tu CK, Paranawithana SR, Tanhauser SM, LoGrasso PV, Laipis PJ, Silverman DN. Enhancement of the catalytic properties of human carbonic anhydrase III by site-directed mutagenesis.Biochemistry. 1991 Feb 12 30(6):1484-90PubMed ID1899618 |
| Method | Catalysis by wild-type HCA III and mutants was determined from the initial velocity of hydration of CO2 at steady state by stopped-flow spectrophotometry and from the exchange of 18O between CO2 and water at chemical equilibrium by mass spectrometry. |
| Comments | at 25°C |
| Entered by | Uri M |
| ID | 102665 |