Diameter of oocyte in graaffian stage

Value 72.3 µm Range: Graph - link µm
Organism Mouse Mus musculus
Reference Griffin J, Emery BR, Huang I, Peterson CM, Carrell DT. Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human). J Exp Clin Assist Reprod. 2006 Mar 13:2.PubMed ID16509981
Primary Source van den Hurk R, Zhao J. Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. Theriogenology. 2005 Apr 163(6):1717-51PubMed ID15763114
Method The accurate calculation of diameters was ensured by using the integrated measuring tools in the Image J software after calibration with a stage micrometer (Gurley Precision Instruments, Troy, NY). Additionally, when measuring diameters, two measurements were taken. The second measurement originated at a right angle from the midpoint of the first measurement (Fig 1c). The two measurements were averaged and expressed as the diameter of the structure.
Comments The graph has values for the oocyte in different stages. Oocytes without a zona pellucida and up to one layer of flattened granulosa progenitor cells were classified as primordial follicles. Primary follicles were classified as oocytes surrounded by one layer of cuboidal granulosa cells. Oocytes with two or more layers of granulosa cells but no visible space between granulosa cells were identified as preantral follicles. Antral follicles, those containing any antral cavity, were further divided into categories of incipient and small antral and Graafian follicles. The incipient follicles, which indicate the beginning of antral formation, were identified by the presence of visible space between granulosa cells. Small antral follicles were identified by the presence of a segmented cavity with two or more compartments, while the Graafian follicles contained one large continuous antral cavity.
Entered by Uri M
ID 102237