| Value |
>=2
kb
Range: The recommended total length of two flanking homologous sequences is 6-10 kb kb
|
| Organism |
Mouse Mus musculus |
| Reference |
Vega biolab-Partner for gene targeting 3701 Market Street, Philadelphia, PA 19104 link |
| Comments |
"FAQs: Q. What is the required minimum length of homologous region?
A. minimum of 2 kb on each homologous arm is recommended. Total of 6-10 kb homologous genomic DNA should give reasonably satisfactory result. If your gene targeting facility has different requirements, [researchers] can make the design accordingly to accommodate the requirements." Regions of homology between the targeting vector and the target locus should be as large as possible to maximize gene targeting efficiency. |
| Entered by |
Irina |
| ID |
101987 |