| Value |
-46.5
kJ/mol
Range: Table - link kJ/mol
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Quang Hon Tran, Gottfried Unden. Changes in the proton potential and the cellular energetics of Escherichia coli during growth by aerobic and anaerobic respiration or by fermentation. European Journal of Biochemistry. 1998. 251(1-2) pp. 538-543 p.540 table 1PubMed ID9492330
|
| Method |
P.540 note above table 1: "Growth was performed in supplemented M9 medium with glycerol (20 mM for growth under oxic, 40 mM for growth under anoxic conditions) or glucose (20 mM) and the electron acceptors given (50 mM, except for O2). In the logarithmic growth phase, samples were drawn and the bacteria were assayed for ATP and ADP. Pi was taken as 20 µmol/g dry cells for growth under oxic and anoxic conditions corresponding to 10 mM (Rao et al., 1993 Shulman et al., 1979 Willsky and Malamy, 1976). ΔG'Phos was calculated from ΔG'Phos = 30.5 kJ/mol 1 RT ln[ATP]/([ADP]×[Pi])." |
| Comments |
P.540 right column bottom paragraph: "ΔG'Phos for growth with various electron acceptors. The phosphorylation potential (ΔG'Phos) is a measure for the energetic status of the bacterial cell. ΔG'Phos was calculated from the contents of ATP, ADP and Pi for E. coli grown with various substrates (Table 1). For the intracellular Pi content, 20 µmol/g dry cells was used throughout. The value is maintained rather constant for variations in the external Pi concentrations or growth conditions due to regulation of Pi uptake (Kashket, 1982: Rao et al., 1993: Shulman et al., 1979: Willsky and Malamy, 1976). For growth by aerobic respiration, the highest ATP contents were found (Table 1)." The number has been converted from positive to negative so that it describes the Gibbs free energy of hydrolysis as opposed to the phosphorylation potential. The E. coli cells were growing without oxygen. See BNID 101701 |
| Entered by |
Phil Mongiovi |
| ID |
101964 |