| Value |
0.687
nmol of H+/min/mg yeast
Range: ±0.077 nmol of H+/min/mg yeast
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Holyoak et al. Activity of the plasma membrane H(+)-ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid. 1996. Appl Environ Microbiol. 62(9): p.3162.PubMed ID8795204
|
| Method |
determined from the rate of proton extrusion into unbuffered water. |
| Comments |
at pH 5.7 in late-exponential phase growth at 30 degrees C. This proton flux is primarily, if not completely through the plasma membrane H+ ATPase. Approximating the mass of 1 cell as 60pg, there are about 17 million yeast cells in 1 mg. The calculation 0.687nmol*6.02E23/17 million gives a value of about 24 million H+ per cell per min, or 400,000 H+ per cell per second. |
| Entered by |
Phil Mongiovi |
| ID |
101902 |