| Value |
237
glucose molecules/sec
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Ye L, Berden JA, van Dam K, Kruckeberg AL. Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae. Yeast. 2001 Sep 30 18(13):1257-67. p.1265 left column 2nd paragraphPubMed ID11561293
|
| Method |
"The cellular abundance of the Hxt7::GFP chimera and the glucose transport activity mediated by that protein were used to calculate the catalytic centre activity of Hxt7 for glucose." "For the second time point (diauxic shift), the Hxt7::GFP cell suspension contained 0.57 mg total cell protein/ml, giving a relationship of 7.0 pmol Hxt7::GFP/mg total cell protein. The Vmax for glucose transport of these cells, 99 nmoles/min/mg total cell protein, was divided by this value, resulting in an estimate of 237/s for the catalytic centre activity of Hxt7::GFP." |
| Comments |
The cellular abundance of the Hxt7::GFP chimera
and the glucose transport activity mediated by
that protein were used to calculate the catalyticcentre
activity of Hxt7 for glucose. For the second time point (diauxic shift), the
Hxt7::GFP cell suspension contained 0.57 mg total
cell protein/ml, giving a relationship of 7.0 pmol
Hxt7::GFP/mg total cell protein. The Vmax for
glucose transport of these cells, 99 nmoles/min/mg
total cell protein, was divided by this value,
resulting in an estimate of 237/s for the catalytic centre
activity of Hxt7::GFP. See BNID 101737, 101739 |
| Entered by |
Ben Marks |
| ID |
101738 |