| Value |
197
glucose molecules/sec
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Ye L, Berden JA, van Dam K, Kruckeberg AL. Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae. Yeast. 2001 Sep 30 18(13):1257-67. p.1265 left column 2nd paragraphPubMed ID11561293
|
| Method |
"Glucose uptake was assayed as described (Walsh
et al., 1994) at five concentrations of D-U-
(14C)-glucose [1, 2.5, 5, 10 and 25 mM (371, 297,
148, 74 and 30 MBq/mol, respectively)]. The data
were fit to the Michaelis–Menten equation using
ENZFITTER software (Elsevier-Biosoft). Total cell
protein was measured by the method of Lowry
(Lowry et al., 1951) using a COBAS Auto-analyzer
(Roche) after digestion of cells overnight in 1 N
NaOH. Bovine serum albumin was used as a
standard. Cell number was determined by counting
at least 1000 cells with a haemocytometer." |
| Comments |
"The cellular abundance of the Hxt7::GFP chimera
and the glucose transport activity mediated by
that protein were used to calculate the catalytic centre
activity of Hxt7 for glucose. For the first
time point (mid-exponential phase), the Hxt7::GFP
cell suspension contained 0.61 mg total cell protein/
ml, giving a relationship of 4.5 pmol Hxt7::GFP/mg
total cell protein. The Vmax for glucose transport of
these cells, 53 nM/min/mg total cell protein, was
divided by this value, resulting in an estimate of
197/s for the catalytic-centre activity of Hxt7::GFP." See BNID 101737, 101738 |
| Entered by |
Ben Marks |
| ID |
101737 |