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rate difference for restriction enzyme EcoRV endonuclease activity for cognate and next best site (ideal conditions)

Value	1e+6 unitless
Organism	Generic
Reference	link
Primary Source	Vermote, C.L. and Halford, S.E. (1992) EcoR V restriction endonuclease: Communication between catalytic metal ions and DNA recognition. Biochemistry 31, 6082–9.
Comments	under ideal conditions. However, under nonoptimal conditions, the differences in cleavage rates between cognate and next-best sites change dramatically for many enzymes. This loss of fidelity or increase in cleavage at sites similar to the cognate site is commonly referred to as star activity.
Entered by	Ron Milo - Admin
ID	101486