Concentration of dATP in oocyte extracts from Xenopus laevis
Range | 5-10 μM |
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Organism | African clawed frog Xenopus laevis |
Reference | Cox LS, Leno GH. Extracts from eggs and oocytes of Xenopus laevis differ in their capacities for nuclear assembly and DNA replication. J Cell Sci. 1990 Sep97 (Pt 1):177-84. p.178 right column 2nd paragraphPubMed ID2258387 |
Method | isotope dilution analysis |
Comments | P.178 right column 2nd paragraph: "Oocyte extracts have been shown by isotope dilution analysis to contain 5-10µM dATP (L.S.C., unpublished results), hence all oocyte extracts were supplemented with 45µM dNTPs to bring precursor pools up to the levels reported in egg extracts (i.e. 50µM: dATP, Blow and Laskey, 1986, dCTP, Hutchison et al. 1987)." |
Entered by | Paul Jorgensen |
ID | 101451 |