| Value |
50
µM
|
| Organism |
African clawed frog Xenopus laevis |
| Reference |
Hutchison CJ, Cox R, Drepaul RS, Gomperts M, Ford CC. Periodic DNA synthesis in cell-free extracts of Xenopus eggs. EMBO J. 1987 Jul6(7):2003-10. p.2007 right column top paragraphPubMed ID3653080
|
| Method |
isotope dilution analysis |
| Comments |
P.2007 left column bottom paragraph: "The amount of DNA synthesized in egg extracts was calculated from material recovered from CsCl gradients. The precursor pool size in egg extracts was estimated to be 50 µM, with respect to
dCTP, by isotope dilution using [3H]dCTP and cold deoxynucleotide triphosphates in a DNA polymerase a assay. Table II shows that for high concentrations of nuclei up to 80% of the template was replicated in 4 h (unsubstituted DNA), whereas at lower starting concentrations of DNA some 200% of the template was replicated. These data strongly support the view proposed by Blow and Laskey (1986) that this synthesis results from efficient initiation of semi-conservative replication." |
| Entered by |
Paul Jorgensen |
| ID |
101450 |