| Value |
20
Unitless
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Aris, J.P. & Blobel G. Isolation of yeast nuclei. Meth. Enz. 194:735 (1991) p.741 2nd paragraphPubMed ID2005821
|
| Primary Source |
[22] O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193 (1951), p. 265 [23] G. Ceriotti, Determination of nucleic acids in animal tissues. J. Biol. Chem., 214 (1955), p. 59PubMed ID14907713, 14367363
|
| Comments |
P.741 2nd paragraph: "A simple way to evaluate the isolation of nuclei is to view the nuclei directly in the Ficoll suspension by light microscopy. Nuclei appear dark, with the crescent-shaped nueleolus sometimes evident (Fig. 1c). Membrane(s) associated with nuclei may be visible (Fig. 1c, lower left). Electron microscopy reveals that nuclei isolated from two Ficoll gradients are substantially free of contamination (Fig. 1a,b). [Investigators] have routinely observed smooth membrane in contact with nuclei and believe this may reflect regions of intimate contact between the nucleus and vacuole, which are often seen in micrographs of yeast cells and spheroplasts (not shown). The protein-to-DNA mass ratio of this preparation is approximately 20:1 as determined by standard methods (primary sources)." |
| Entered by |
Paul Jorgensen |
| ID |
101431 |