Doubling time of haploid cell

Value 99 minutes Range: ±1 Table - link minutes
Organism Budding yeast Saccharomyces cerevisiae
Reference Talia, S. D., J. M. Skotheim, J. M. Bean, E. D. Siggia, and F. R. Cross, 2007. The effects of molecular noise and size control on variability in the budding yeast cell cycle. Nature 448:947–951. DOI: 10.1038/nature06072 Supplementary information p. 20 table S12PubMed ID17713537
Method P.947 left column bottom paragraph: "[Researchers] measured times from cytokinesis to budding (G1) and from budding to cytokinesis in haploids, diploids or tetraploids (mothers and daughters), using time-lapse fluorescence microscopy of strains expressing Myo1 tagged with green fluorescent protein (Myo1–GFP)."
Comments P.947 right column 3rd paragraph: "When [researchers] quantified total red fluorescence per cell as described [ref 24], [they] found exponential growth in single cells (Fig. 2a Supplementary Information), as deduced previously from pulse-labelling of size-selected populations [ref 25]. The single-cell growth rate α is moderately variable, but its average agrees well with the bulk culture growth rate (Fig. 2b, Supplementary Table 12)." Media and temperature dependent. Although unspecified, the growth media appears to be glucose according to following sentence from p. 16 in supplementary information (bottom paragraph): "Glycerol/ethanol supports a much slower growth rate than glucose (170 min compared to 100 min doubling time)..." For doubling time of haploid mother cell from the same article see BNID 104360. For 100 min in rich medium see BNID 100270.
Entered by Ben Marks
ID 101310