| Value |
0.0057
µM
Range: Table - link µM
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. Fujioka A, Terai K, Itoh RE, Aoki K, Nakamura T, Kuroda S, Nishida E, Matsuda M. J Biol Chem. 2006 Mar 31 281(13):8917-26PubMed ID16418172
|
| Primary Source |
Schoeberl B, Eichler-Jonsson C, Gilles ED, Müller G. Computational modeling of the dynamics of the MAP kinase cascade activated by surface and internalized EGF receptors. Nat Biotechnol. 2002 Apr20(4):370-5 Table - link PubMed ID11923843
|
| Method |
(Primary source) A computational model that offers an integrated quantitative, dynamic, and topological representation of intracellular signal networks, based on known components of epidermal growth factor (EGF) receptor signal pathways. |
| Comments |
This value is from table link in reference. It seems to be taken from primary source table link value of 4×10^4 Raf/cell given in Primary source table link. However, if calculated according to cell volume of 1 pL (note under ref table link) one arrives at [(4×10^4<Raf/cell>/~6×10^23)<Raf/mole>]/10^12<liter/cell>=~0.067µM, an order of magnitude greater than above value. Above value is half of Raf conc. of 0.013µM given by ref (FRET method), BNID 100854. In silico (performed on computer) kinetic simulation models of the RAS/ERK MAPK pathway, while describing it very nicely, have produced astonishingly different parameters. The aim of study in ref was to collect and evaluate the parameters for the development of a kinetic simulation model for Ras/ERK MAPK cascades. |
| Entered by |
Cellina Cohen-Saidon |
| ID |
100890 |