| Value |
5.5
nN/µm^2
Range: ±2 nN/µm^2
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Balaban NQ et al., Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates. Nat Cell Biol. 2001 May3(5):466-72 abstract and p.469 right column top paragraphPubMed ID11331874
|
| Method |
A method combining micropatterning of elastomer substrates and fluorescence imaging of focal adhesions in live cells expressing GFP-tagged vinculin. |
| Comments |
p.469 left column bottom paragraph:"The relationship between force and area in all focal adhesions at all time points is plotted in Fig. 5e (each symbol represents a different focal adhesion). The correlation seen at t = 0 (Fig. 5a) is now more apparent and the determined linear dependence defines a constant stress, Pf=5.5±2nN/µm^2, applied at the focal adhesions. Extrapolation of this linear dependence to zero force shows that the tension-independent area of focal adhesions is ~µm^2. This residual area is similar to the typical size of focal complexes, which are the precursors of the larger adhesions formed once force is applied [ref 12]." Human foreskin fibroblasts and primary neonatal rat cardiomyocyte and cardiac fibroblasts cell cultures. |
| Entered by |
Ronen Zaidel-Bar |
| ID |
100620 |