Cell total volume

Value 1.1 µm^3 Range: 0.44-1.79 Table - link µm^3
Organism Bacteria Escherichia coli
Reference Kubitschek HE, Friske JA. Determination of bacterial cell volume with the Coulter Counter. J Bacteriol. 1986 Dec168(3):1466-7. p.1466 table 1PubMed ID3536882
Method Coulter counter: A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions. When a particle flows through one of the microchannels, it results in the electrical resistance change of the liquid filled microchannel. This resistance change can be recorded as electric current or voltage pulses, which can be correlated to size, mobility, surface charge and concentration of the particles. Cells of several different sizes were obtained from either stationary- or exponential-phase cultures of E. coli B/rA grown overnight at 37°C in a shaker water bath in nutrient broth or M9-glucose (10 g/liter) medium. Culture volumes were 160 ml for stationary-phase cultures and 50 ml for exponentially growing cultures.
Comments P.1466 right column top paragraph: "Because the specific activities appear only as ratios in the calculations of amounts of interstitial fluid, the errors in these calculations can be no more than about 0.25%, much less than the observed experimental errors (Table 1)." P.1467 left column top paragraph: "Uncorrected pellet volumes usually were about 0.4 to 0.5 ml, and the average values calculated for the interstitial fluid volumes varied from about 25 to 35% of the pellet volume (Table 1)." Value is average of 11 measurements in table link. See BNID 101788, 104825
Entered by Ron Milo - Admin
ID 100004