| Value |
6.9
Hours
|
| Organism |
Human Homo sapiens |
| Reference |
Eden E, Geva-Zatorsky N, Issaeva I, Cohen A, Dekel E, Danon T, Cohen L, Mayo A, Alon U. Proteome half-life dynamics in living human cells. Science. 2011 Feb 11 331(6018):764-8 p.766 figure 2C,DPubMed ID21233346
|
| Method |
Researchers present an assay called “bleach-chase” for measuring protein half-lives that does not require translation inhibitors, antibodies, or radioactive labeling. In the bleach-chase method, the fluorophore of the tagged protein is bleached using a brief pulse of light, irreversibly rendering it nonfluorescent. Half life obtained using the equation on p.765 under fig.1, half life=ln(2)/alpha taking average alpha as 0.1 (p.766 note under fig.2D) |
| Comments |
Note, in p.765 middle column bottom paragraph a mean value of 9 hours is given: "[Researchers] observed a broad protein half-life distribution ranging between 45 min to 22.5 hours, with mean 9.0±4.6 hours (Fig. 2D and table S4)." H1299 human non–small cell lung cancer cell line. |
| Entered by |
Uri M |
| ID |
106875 |