Copy number of plasmids with ColE1 origin of replication
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|Organism||Bacteria Escherichia coli|
|Reference||Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15 25(6):1203-10 p.1205 table 1PubMed ID9092630|
|Method||The intracellular copy numbers were determined by comparing luciferase activity of cells harbouring the respective plasmids with the activity in cells containing only a single luciferase expression unit integrated in the chromosome (data not shown)|
|Comments||Module II contains an origin of replication which is protected from outside transcriptional readthrough (ref 14 ) by two terminators. See figure 2|
|Entered by||Uri M|