Cell total volume
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| Value | 1.1 µm^3 Range: 0.44-1.79 Table-http://bionumbers.hms.harvard.edu/files/E.%20coli%20cell%20volume%20in%20different%20media%20in%20stationary%20and%20exponential%20phase.pdf µm^3 |
|---|---|
| Organism | Bacteria Escherichia coli |
| Reference | Kubitschek HE, Friske JA. Determination of bacterial cell volume with the Coulter Counter. J Bacteriol. 1986 Dec168(3):1466-7. p.1466 table 1PubMed ID3536882 |
| Method | Coulter counter: A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions. When a particle flows through one of the microchannels, it results in the electrical resistance change of the liquid filled microchannel. This resistance change can be recorded as electric current or voltage pulses, which can be correlated to size, mobility, surface charge and concentration of the particles. Cells of several different sizes were obtained from either stationary- or exponential-phase cultures of E. coli B/rA grown overnight at 37°C in a shaker water bath in nutrient broth or M9-glucose (10 g/liter) medium. Culture volumes were 160 ml for stationary-phase cultures and 50 ml for exponentially growing cultures. |
| Comments | Value is average of 10 measurements in table link. See BNID 101788, 104825, 103690 |
| Entered by | Ron Milo - Admin |
| ID | 100004 |